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KMID : 1149220140220010079
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Korean Journal of Oriental Medical Prescription
2014 Volume.22 No. 1 p.79 ~ p.92
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Anti-oxidative and anti-inflammatory experiments of Talmyung-san in RAW264.7 cells
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Cho Hyun-Jin
Park Sun-Dong
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Abstract
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Objectives : The aim of this study was identification of the anti-oxidative and anti-inflammatory effects of Talmyung-san (TMS) in mouse macrophage, RAW264.7 cells.
Methods : To identify the anti-oxidative effect of TMS, scavenging activities of DPPH radical, nitric oxide and peroxynitrite were measured in vitro. In RAW264.7 cells, DCFH-DA assay was conducted to examine the inhibitory effect of TMS on ROS production in response to lipopolysaccharide. And the productions of nitric oxide (NO), PGE2 and pro-inflammatory cytokines were measured. The levels of COX-2, iNOS, nuclear NF-κB p65 expression and phosphorylation of IκB-α in cytosol were detected by western blotting analyses.
Results : TMS couldn’t scavenged DPPH radical, but nitric oxide and peroxynitrite were decreased. TMS decreased intracellular ROS, NO, and IL-1β production effectively. However, TMS inhibited PGE2 levels only in high concentration (300 ㎍/㎖) and TMS failed to suppress the production of IL-6 and TNF-α. Results from immunoblot analyses revealed that TMS decreased activation of COX-2, iNOS, phosphorylation of IκB-α and nuclear translocation of p65.
Conclusions : TMS has anti-RNS and anti-inflammatory effects via NF-κB pathway and more intensive studies will be required to evaluate therapeutic potential of TMS.
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KEYWORD
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Talmyung-san, anti-inflammation, anti-RNS
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